Lake City is the county seat of Columbia County, Florida, United States. As of the 2010 census, the city's population was 12,046. It is the principal city of the Lake City Micropolitan Statistical Area, which is composed of Columbia County, and had a 2010 population of 67,531.
Lake City first began as the town of Alligator in 1821 near the Native American settlement Alligator Village. Alligator became the seat of Columbia County in 1832 when it was formed from Duval and Alachua County. In 1858 Alligator was incorporated and renamed Lake City. The largest American Civil War battle in Florida took place near Lake City at the Battle of Olustee in 1864 with a Confederate victory. In 1884 the Florida Agricultural College was established in Lake City until its relocation to Gainesville in 1905 to form part of the University of Florida. The city's sesquicentennialwas held in 2009.
Lake City is known as "The Gateway to Florida" because it is adjacent to the intersection of Interstate 75 and Interstate 10. Lake City is the site of Lake City Gateway Airport formerly known as NAS Lake City. Florida Gateway College is located in Lake City.